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This alcohol is crucial because it inhibits bacterial growth after the vial has been opened and the rubber stopper has been punctured, allowing for multiple uses of the same vial over several weeks

The Ni-NTA bead suspension was transferred to 5-ml disposable chromatography columns (29922, Thermo Fisher Scientific)

Additionally, we reassessed the relationships between prefrontal GABA and CBF levels within the PTSD group after excluding potentially influential data points, further confirming the stability of the original findings