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The system prepared hyaluronic acid/gelatin/sodium alginate hydrogel loaded with NAC-modified gold nanoclusters (NAC-Au NCs) by 3D printing technology (N-Au@hydrogel) and combined with biological hydrogel-encapsulated hepatocyte spheroids containing acellular liver matrix (dECM), thrombin and fibrinogen (HS@dECM) [Figure 4A]

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When staining was performed with anti-GCLC, anti-cleaved caspase3, anti-TSPO (PBR), anti-PSD95 or anti-GSDME antibodies, we applied a fluorescence-indirect tyramide signal amplification (TSA) technique (TSA System

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