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Supporting this, Fedoreyeva and colleagues reported in 2011 that fluorescently labeled short peptides including Epitalon (Ala-Glu-Asp-Gly), Pinealon (Glu-Asp-Arg), and Testagen (Lys-Glu-Asp-Gly) accumulated in the cytoplasm, nucleus, and nucleolus of HeLa cells after incubation, indicating that such peptides can indeed penetrate an animal cell and reach nuclear compartments in vitro

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Furthermore, a greater magnitude of weight loss was associated with a greater increase in total ghrelin concentrations, suggesting a physiological adaptation after weight loss